Plant:- Plant barcoding studies use plastid regions (e.g. rbcL, MatK) and the internal spacer regions(ITS) of nuclear ribosomal DNA.(Sample type;- Fresh plant organs)
Insects:- Animal barcoding studies use a region in the mitochondrial Cytochrome C oxidase 1 gene(COI)(Sample types:- Whole organisms are preferable)
Fungus:-Fungal barcoding studies use the internal transcribed spacer regions(ITS) in the nuclear ribosomal cistron. This region shows reasonable discriminatory power at the species level in many groups.
Work flow at lab:-
Step1: Isolate DNA from the sample
Step2:- Amplify the target DNA barcode region using PCR
Step4: Compare the resulting sequence against reference database to find the matching species using appropriate softwares.
Report:- Report of the test will be dispatched to the forwarding authority directly within 7 working days. We are here to assist you at any stage of study.