Plant identification is very important aspect in the field of herbal or natural products like herbal medicines and cosmetics. Accurate identification of cultivated plant can be very helpful for horticulturist in knowing how it grows as well as how to care and protect it from pests and disease Plants can be sometimes hard to distinguish on basis of morphological characters for an untrained eyes.DNA based methods now provide essential tools for accurate plant identification and disease diagnosis. The dramatic advancement in molecular genetics helps researchers and workers by providing easy and reliable techniques of plant identification. Molecular techniques such as DNA barcoding, random amplified polymorphic DNA (RAPD) , amplified fragment length polymorphism (AFLP) , microsatellite and single nucleotide polymorphism (SNP) are commonly used methods enable to acquired one or more dna sequences . Each technique has its own advantages and limitations.
Currently, dye terminator sequencing is the standard method is sequence analysis .In dye terminator sequencing, the four district nucleotide chain terminators were labeled with fluorescent dyes with different fluorescence emission wavelengths.
The main Advantages of Sanger sequencing based sequencing are its automation, robustness and higher accuracy. but it inable to handle long sequences however it can reliably sequence up to approximately 900 nucleotide long DNA fragments in a single reaction. Typing of nucleotide sequences such as maturaseK (matK) and ribulose-1, 5-bisphosphate carboxylase (rbcL) and ITS marker loci which are commonly used in plant species identification.
GeneOmbio offers conventional PCR based plant identification and bioinformatics services which helps researchers in appropriate identification and characterization of plant materials which is essential for the successful conservation of plant resources and to ensure their sustainable use.