Ploidy Analysis

What is Ploidy and how it analyzed? Ploidy is the molecular biology term used for the number of basic set of chromosome in the nucleus of the any cell. Level of ploidy is number of chromosome set s in somatic cells of the diploid phase or haploid phase. These set of the chromosome are required for studies related to plant analysis.

Generally ploidy levels of plants can be measured using stained chromosomes with the microscope in which no of chromosomes are measured. And widely used or standard method for ploidy analysis is flow cytometry. In flow cytometry usually fresh sample required for the analysis. At geneOmbio technologies ploidy analysis services are provided. A well advanced flow Cytometer from Thermo provides accurate and fast data for ploidy analysis. Ploidy can be assessed by chromosome number or flow cytometry. At GBL ploidy level is analyzed by Flow cytometry.

 

Basically, a flow Cytometer is a fluorescence microscope which analyses moving particles in a suspension. These are excited by a source of light it can be UV light or laser. In turn emit an fluorescence which is filtered through a series of dichroic mirrors. Then, the inbuilt programme of the equipment converts these signals into a graph plotting the intensity of the fluorescence emitted against the count of cells emitting it at a time given. Thus, a flow cytometer consists of fluidics, optics and electronics, as it measures cells in suspension that flow in single-file through an illuminated volume where they scatter light and emit a fluorescence that is collected, filtered and converted to digital values for storage on a computer. This gives advantage of accuracy, specificity of the results